Aberrant microRNA expression in the brains of neurodegenerative diseases: miR‐29a decreased in Alzheimer disease brains targets neurone navigator 3
Identifieur interne : 000831 ( Main/Exploration ); précédent : 000830; suivant : 000832Aberrant microRNA expression in the brains of neurodegenerative diseases: miR‐29a decreased in Alzheimer disease brains targets neurone navigator 3
Auteurs : M. Shioya ; S. Obayashi ; H. Tabunoki ; K. Arima ; Y. Saito ; T. Ishida [Japon] ; J. SatohSource :
- Neuropathology and Applied Neurobiology [ 0305-1846 ] ; 2010-06.
English descriptors
- KwdEn :
Abstract
M. Shioya, S. Obayashi, H. Tabunoki, K. Arima, Y. Saito, T. Ishida and J. Satoh (2010) Neuropathology and Applied Neurobiology36, 320–330
Aberrant microRNA expression in the brains of neurodegenerative diseases: miR‐29a decreased in Alzheimer disease brains targets neurone navigator 3 Aims: MicroRNAs (miRNAs) are small non‐coding RNAs that regulate translational repression of target mRNAs. Accumulating evidence indicates that various miRNAs, expressed in a spatially and temporally controlled that manner in the brain plays a key role in neuronal development. However, at present, the pathological implication of aberrant miRNA expression in neurodegenerative events remains largely unknown. To identify miRNAs closely associated with neurodegeneration, we performed miRNA expression profiling of brain tissues of various neurodegenerative diseases. Methods: We initially studied the frontal cortex derived from three amyotrophic lateral sclerosis patients by using a microarray of 723 human miRNAs. This was followed by enlargement of study population with quantitative RT‐PCR analysis (n = 21). Results: By microarray analysis, we identified up‐regulation of miR‐29a, miR‐29b and miR‐338‐3p in amyotrophic lateral sclerosis brains, but due to a great interindividual variation, we could not validate these results by quantitative RT‐PCR. However, we found significant down‐regulation of miR‐29a in Alzheimer disease (AD) brains. The database search on TargetScan, PicTar and miRBase Target identified neurone navigator 3 (NAV3), a regulator of axon guidance, as a principal target of miR‐29a, and actually NAV3 mRNA levels were elevated in AD brains. MiR‐29a‐mediated down‐regulation of NAV3 was verified by the luciferase reporter assay. By immunohistochemistry, NAV3 expression was most evidently enhanced in degenerating pyramidal neurones in the cerebral cortex of AD. Conclusions: These observations suggest the hypothesis that underexpression of miR‐29a affects neurodegenerative processes by enhancing neuronal NAV3 expression in AD brains.
Url:
DOI: 10.1111/j.1365-2990.2010.01076.x
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Aberrant microRNA expression in the brains of neurodegenerative diseases: miR‐29a decreased in Alzheimer disease brains targets neurone navigator 3</title><author><name sortKey="Shioya, M" sort="Shioya, M" uniqKey="Shioya M" first="M." last="Shioya">M. Shioya</name></author><author><name sortKey="Obayashi, S" sort="Obayashi, S" uniqKey="Obayashi S" first="S." last="Obayashi">S. Obayashi</name></author><author><name sortKey="Tabunoki, H" sort="Tabunoki, H" uniqKey="Tabunoki H" first="H." last="Tabunoki">H. Tabunoki</name></author><author><name sortKey="Arima, K" sort="Arima, K" uniqKey="Arima K" first="K." last="Arima">K. Arima</name></author><author><name sortKey="Saito, Y" sort="Saito, Y" uniqKey="Saito Y" first="Y." last="Saito">Y. Saito</name></author><author><name sortKey="Ishida, T" sort="Ishida, T" uniqKey="Ishida T" first="T." last="Ishida">T. Ishida</name></author><author><name sortKey="Satoh, J" sort="Satoh, J" uniqKey="Satoh J" first="J." last="Satoh">J. Satoh</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:9362C1A83BE2B83371BC3E4CB39068428AC2AE32</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1111/j.1365-2990.2010.01076.x</idno><idno type="url">https://api.istex.fr/document/9362C1A83BE2B83371BC3E4CB39068428AC2AE32/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">002185</idno><idno type="wicri:Area/Main/Curation">001E76</idno><idno type="wicri:Area/Main/Exploration">000831</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Aberrant microRNA expression in the brains of neurodegenerative diseases: miR‐29a decreased in Alzheimer disease brains targets neurone navigator 3</title><author><name sortKey="Shioya, M" sort="Shioya, M" uniqKey="Shioya M" first="M." last="Shioya">M. Shioya</name><affiliation><wicri:noCountry code="subField"></wicri:noCountry></affiliation></author><author><name sortKey="Obayashi, S" sort="Obayashi, S" uniqKey="Obayashi S" first="S." last="Obayashi">S. Obayashi</name><affiliation><wicri:noCountry code="subField"></wicri:noCountry></affiliation></author><author><name sortKey="Tabunoki, H" sort="Tabunoki, H" uniqKey="Tabunoki H" first="H." last="Tabunoki">H. Tabunoki</name><affiliation><wicri:noCountry code="subField"></wicri:noCountry></affiliation></author><author><name sortKey="Arima, K" sort="Arima, K" uniqKey="Arima K" first="K." last="Arima">K. Arima</name><affiliation><wicri:noCountry code="subField"></wicri:noCountry></affiliation></author><author><name sortKey="Saito, Y" sort="Saito, Y" uniqKey="Saito Y" first="Y." last="Saito">Y. Saito</name><affiliation><wicri:noCountry code="subField">and</wicri:noCountry></affiliation></author><author><name sortKey="Ishida, T" sort="Ishida, T" uniqKey="Ishida T" first="T." last="Ishida">T. Ishida</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Kohnodai Hospital, International Medical Center, Chiba</wicri:regionArea><wicri:noRegion>Chiba</wicri:noRegion></affiliation></author><author><name sortKey="Satoh, J" sort="Satoh, J" uniqKey="Satoh J" first="J." last="Satoh">J. Satoh</name><affiliation><wicri:noCountry code="subField"></wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j">Neuropathology and Applied Neurobiology</title><idno type="ISSN">0305-1846</idno><idno type="eISSN">1365-2990</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2010-06">2010-06</date><biblScope unit="volume">36</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="320">320</biblScope><biblScope unit="page" to="330">330</biblScope></imprint><idno type="ISSN">0305-1846</idno></series><idno type="istex">9362C1A83BE2B83371BC3E4CB39068428AC2AE32</idno><idno type="DOI">10.1111/j.1365-2990.2010.01076.x</idno><idno type="ArticleID">NAN1076</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0305-1846</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alzheimer disease</term><term>bioinformatics</term><term>miR‐29a</term><term>microarray</term><term>neurone navigator 3</term><term>real‐time RT‐PCR</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">M. Shioya, S. Obayashi, H. Tabunoki, K. Arima, Y. Saito, T. Ishida and J. Satoh (2010) Neuropathology and Applied Neurobiology36, 320–330
Aberrant microRNA expression in the brains of neurodegenerative diseases: miR‐29a decreased in Alzheimer disease brains targets neurone navigator 3 Aims: MicroRNAs (miRNAs) are small non‐coding RNAs that regulate translational repression of target mRNAs. Accumulating evidence indicates that various miRNAs, expressed in a spatially and temporally controlled that manner in the brain plays a key role in neuronal development. However, at present, the pathological implication of aberrant miRNA expression in neurodegenerative events remains largely unknown. To identify miRNAs closely associated with neurodegeneration, we performed miRNA expression profiling of brain tissues of various neurodegenerative diseases. Methods: We initially studied the frontal cortex derived from three amyotrophic lateral sclerosis patients by using a microarray of 723 human miRNAs. This was followed by enlargement of study population with quantitative RT‐PCR analysis (n = 21). Results: By microarray analysis, we identified up‐regulation of miR‐29a, miR‐29b and miR‐338‐3p in amyotrophic lateral sclerosis brains, but due to a great interindividual variation, we could not validate these results by quantitative RT‐PCR. However, we found significant down‐regulation of miR‐29a in Alzheimer disease (AD) brains. The database search on TargetScan, PicTar and miRBase Target identified neurone navigator 3 (NAV3), a regulator of axon guidance, as a principal target of miR‐29a, and actually NAV3 mRNA levels were elevated in AD brains. MiR‐29a‐mediated down‐regulation of NAV3 was verified by the luciferase reporter assay. By immunohistochemistry, NAV3 expression was most evidently enhanced in degenerating pyramidal neurones in the cerebral cortex of AD. Conclusions: These observations suggest the hypothesis that underexpression of miR‐29a affects neurodegenerative processes by enhancing neuronal NAV3 expression in AD brains.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Arima, K" sort="Arima, K" uniqKey="Arima K" first="K." last="Arima">K. Arima</name><name sortKey="Obayashi, S" sort="Obayashi, S" uniqKey="Obayashi S" first="S." last="Obayashi">S. Obayashi</name><name sortKey="Saito, Y" sort="Saito, Y" uniqKey="Saito Y" first="Y." last="Saito">Y. Saito</name><name sortKey="Satoh, J" sort="Satoh, J" uniqKey="Satoh J" first="J." last="Satoh">J. Satoh</name><name sortKey="Shioya, M" sort="Shioya, M" uniqKey="Shioya M" first="M." last="Shioya">M. Shioya</name><name sortKey="Tabunoki, H" sort="Tabunoki, H" uniqKey="Tabunoki H" first="H." last="Tabunoki">H. Tabunoki</name></noCountry><country name="Japon"><noRegion><name sortKey="Ishida, T" sort="Ishida, T" uniqKey="Ishida T" first="T." last="Ishida">T. Ishida</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000831 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000831 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= ParkinsonV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:9362C1A83BE2B83371BC3E4CB39068428AC2AE32
|texte= Aberrant microRNA expression in the brains of neurodegenerative diseases: miR‐29a decreased in Alzheimer disease brains targets neurone navigator 3
}}
| This area was generated with Dilib version V0.6.23. |  |